Monoclonal antibody SMI 99 against myelin basic protein
产品名称： Monoclonal antibody SMI 99 against myelin basic protein
英文名称： Monoclonal antibody SMI 99 against myelin basic protein
产品编号： SMI-99P
产品价格： null
产品产地： USA
品牌商标： COVANCE
更新时间： 2024-12-10T11:28:44
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
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| Description |  |
Monoclonal antibody SMI 99 against myelin basic protein |
| Intended Use | |
**Research Use Only (RUO)** This product is sold for laboratory research use only, not for human or in-vivo use. |
| Clone | |
SMI-99 |
| Form | |
Purified Antibody (contains 0.01 M sodium azide) |
| Host | |
Mouse |
| Species Reactivity | |
Mammalian |
| IsoType | |
IgG2b |
| [Ab] | |
1 mg/mL |
| Specificity | |
SMI 99 reacts with the sequence Ala-Ser-Asp-Tyr-Lys-Ser in position 131-136 of human myelin basic protein. The antibody detects myelin basic protein from most mammalian species. Pig and chicken MBP do not react and guinea pig MBP has slight reactivity. SMI 99 does not react with the 14 Kda form of rat MBP. The antibody isotype is IgG2b. SMI 99 detects developing and adult myelin, developing oligodendrocytes and distinguishes oligodendrocytes from astrocytes, microglia, neurons and other cells in brain sections. The combination of SMI 99 with SMI 91 and/or 94 is useful for immunocytochemical studies on the progression of normal and pathologic myelination. SMI-99 is DEAE-purified IgG from mouse ascites. |
| Uses | |
This antibody is effective in immunoblotting, immunocytochemistry (ICC) and ELISA. |
| Suggested Working Dilution | |
The optimal working dilution should be determined for each specific assay condition.
The extent of permissible dilution of SMI 99 beyond those recommended for general application depends upon nature and concentration of the antigen examined, species of the antigen, method of fixation and kind of section examined. |
| Storage | |
Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C. |
| References | |
Mendez G, Moore WJ, Easterbrook-Smith, Linthicum DS. A protein NMR study of interaction of myelin basic protein with a monoclonal antibody. Biochem J 228:61-68, 1985. DeRosbo NK, Carnegie PR, Bernard CCA, Linthicum DS. Detection of various forms of brain myelin basic protein in vertebrates by electroimmunoblotting. Neurochem Res 9:1359-1369, 1984. Carnegie PR, Dowse CA, Linthicum DS. Antigenic determinant recognized by a monoclonal antibody to human myelin basic protein. J Neuroimmunol 5:125-134, 1983. Dowse CA, Carnegie PR, Linthicum DS, Bernard CCA. Solid phase radioimmunoassay for human myelin basic protein using a monoclonal antibody. J Neuroimmunol 5:135-144, 1983. |
| Warranty/Conditions | |
Covance products may not be resold or modified for resale without prior written approval. |
| Rev. Date | |
11/24/2008 |