Product: Anti-Nitric Oxide Synthase-1 mAb, clone NOS-125
Cat. No.: MC-129 (1 mL, lyophilized)
Synonyms:
Neuronal NOS, nNOS
Background:
Human nitric oxide synthases are a family of
enzymes responsible for the synthesis of nitric
oxide from L-argenine and molecular oxygen.
There are at least three nitric oxide synthases;
NOS I, also known as neuronal NOS or nNOS,
NOS II, which is referred to as inducible NOS or
iNOS and NOS III, also known as endothelial
NOS or eNOS. As suggested by their
nomenclature, these enzymes have different
cellular distributions and are subjected to different
regulatory mechanisms. NOS III, like NOS I, is a
constitutive form of NOS and produces picomolar
quantities of nitric oxide (NO) which plays a role
in signal transmission and results in physiological
effects. In the gastrointestinal tract, NO plays a
protective role where it has direct microbiocidal
properties and acts as a first line of mucosal
defense against luminal organisms in the
stomach.
Species Reactivity:
Human
Ig Isotype:
Mouse IgG1
Immunogen:
Prokaryotic recombinant protein corresponding to
a portion of the C-terminus of the for
immunizations mature human nitric oxide
synthase-1 protein.
Staining pattern:
Cytoplasmic
Format:
1 mL lyophilized tissue culture supernatant
containing 15 mM sodium azide.
Positive control:
Bowel
Reconstitution:
Reconstitute with 1 mL sterile distilled water.
Storage:
Unopened lyophilized antibody can be stored at
4°C for short term storage. After reconstitution,
the antibody is stable for at least two months
when stored at 4°C. For long term storage, it is
recommended that aliquots of the antibody be
made and frozen at -20°C (frost-free freezers are
not recommended). Repeated freezing and
thawing must be avoided. Prepare working
dilutions on the day of use.
Applications:

Immunohistochemistry: Paraffin sections.
Use at a 1:25 - 1:50 dilution. High
temperature antigen unmaking technique is
recommended. Use a 60 minute primary
antibody incubation at 25°C. Standard ABC
technique.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.